Correction of Drug-Induced Hepatotoxicity With New Triterpene Derivatives in Transplanted RLS Lymphoma.

Correction of Drug-Induced Hepatotoxicity with New Triterpene Derivatives in Transplanted RLS Lymphoma.

Bull Exp Biol Med. 2012 Dec; 154(3): 370-374
Zhukova NA, Semenov DE, Bessergeneva EP, Sorokina IV, Tolstikova TG

The hepatoprotective effects of new triterpene derivatives, betulin 3?,28-di-O-nicotinate (of3) and 3,20-dioximino-29-norlup-28-ic acid methyl ester (of15), were studied in CBA/Lac mice with transplanted RLS lymphoma receiving polychemotherapy and without it. Injection of of3 and of15 agents to animals with tumors receiving polychemotherapy reduced the severity of toxic involvement of the liver, reduced mitotic activity of tumor cells in the primary node in animals receiving and not polychemotherapy, and produced a moderate antitumor effect. These effects were more pronounced for of15 agent. In addition, injection of agents of3 and of5 to animals with transplanted RLS lymphoma reduced the intensity of alterations associated with the total systems and local effects of the neoplastic process. HubMed – drug

Efficiency of Noopept in Streptozotocin-Induced Diabetes in Rats.

Bull Exp Biol Med. 2012 Dec; 154(3): 334-338
Ostrovskaya RU, Ozerova IV, Gudascheva TA, Kapitsa IG, Ivanova EA, Voronina TA, Seredenin SB

We studied the effects of new nootropic and neuroprotective drug Noopept (N-phenylacetyl-L-prolylglycine ethyl ester) in various dosage regimens on the dynamics of glycemia, body weight, and pain sensitivity in rats receiving diabetogenic toxin streptozotocin. In experimental diabetic rats, Noopept alleviated glycemia and weight loss and normalized enhanced pain sensitivity. The normalizing effect of Noopept was most pronounced when it was administered as a preventive agent prior to injection of the toxin. Both preventive and therapeutic administration of Noopept (delayed injections included) significantly weakened the examined metabolic effects of diabetogenic toxin. Possible mechanisms of the antidiabetic action of Noopept are analyzed. HubMed – drug

Antifibrotic Effect of Combined Treatment with Neuroleptic Drug and Immobilized Hyaluronidase in Pulmonary Fibrosis.

Bull Exp Biol Med. 2012 Dec; 154(3): 329-333
Dygai AM, Skurikhin EG, Ermakova NN, Pershina OV, Krupin VA, Reztsova AM, Ermolaeva LA, Khmelevskaya ES, Stepanova IE, Artamonov AV, Bekarev AA, Madonov PG, Kinsht DN, Goldberg VE, Semiglazova TA

Using the model of lung fibrosis induced by intratracheal administration of bleomycin we studied anti-fibrotic activity of combined treatment with neuroleptic haloperidol and hyaluronidase immobilized on polyethylene oxide using electron-beam synthesis. It was shown that successive administration of immobilized hyaluronidase and the neuroleptic drug inhibits deposition of collagen fibers in the bleomycin-treated lungs. Combined treatment with the test compounds reduced swelling of the alveolar epithelium, exudation and infiltration of the alveolar interstitium and alveolar passages by inflammatory cells, and desquamation of alveolocytes into alveolar lumen, so that the alveolar-capillary membrane function was preserved. HubMed – drug

MDR Gene Expression Analysis of Six Drug-Resistant Ovarian Cancer Cell Lines.

Biomed Res Int. 2013; 2013: 241763
Januchowski R, Wojtowicz K, Sujka-Kordowska P, Andrzejewska M, Zabel M

Ovarian cancer is the leading cause of death among gynaecological malignancies. Multiple drug resistance makes cancer cells insensitive to chemotherapy. In this study, we developed six primary ovarian cancer cell lines (W1MR, W1CR, W1DR, W1VR, W1TR, and W1PR) resistant to drugs such as methotrexate, cisplatin, doxorubicin, vincristine, topotecan, and paclitaxel. A chemosensitivity assay MTT test was performed to assess drug cross-resistance. Quantitative real-time polymerase chain reaction and Western blot were also performed to determine mRNA and protein expression of genes involved in chemoresistance. We observed high cross-resistance to doxorubicin, vincristine, and paclitaxel in the cell lines resistant to these agents. We also found a significant correlation between resistance to these drugs and increased expression of P-gp. Two different mechanisms of topotecan resistance were observed in the W1TR and W1PR cell lines. We did not observe any correlation between MRP2 transcript and protein levels. Cell lines resistant to agents used in ovarian cancer treatment remained sensitive to methotrexate. The main mechanisms of drug resistance were due to P-gp expression in the doxorubicin, vincristine, and paclitaxel resistant cell lines and BCRP expression in the topotecan resistant cell line. HubMed – drug